Differential Leukocyte Count Lab Method | Differential Blood Count | Leukocyte Differential Count

In this Method We count & Identify Leukocyte Such as Neutrophil, Basophil, Eosinophils, Monocyte. Three stains that used in differential leukocyte count 1-Wright stain, 2-Leishman stain 3-Giemsas stain Method of staining : 1-The blood film is fixed with methyl alcohol for 2 minutes . 2-Pour Giemsa stain diluted 1:9 with buffer over the smear for 8-10 minutes. 3-Wash off with buffer and dry The procedure of slide to slide : 1-Place a drop of blood from the finger about 2mm in diameter in the central line of a slide about 1-2 cm from one end . 2-The spreader is placed at an angle of 40 degrees to the slide and then moved back to make contact with the drop. 3-The drop should spread out quickly along the line of contact of the spreader with the slide. 4-The moment this occurred the film should be spread by a rapid, smooth,forward,movement of spreader. 5-The drop should be of such size that the film is 3-4 cm in length . 6-The film should be dried rapidly .A good blood film preparation will be thick at the drop end and thin film at the opposite end. the thickness of the spread when pulling the smear is determined by the :A-angle of spreader slide (The greater the angle ,The thicker and Shorter the smear ).B-size of the blood drop .C-Speed of spreading (Slowly the thicker).while the thin smears are used for describing blood cell, the thick smear are used for detecting malarial parasites .

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